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Amounts of tanshenoside Ⅰ in cultivated Ludangshen of different years of cultivation history was determined by TLC densitometry in comparison with that in wildly grown Ludangshen and cultivated Taidangshen. Results showed that tanshenoside Ⅰ in cultivated Ludangshen decreases with increased years of cultivation history while that in cultivated Taidangsihen is slightly lower than that of Ludangshen of the same years of cultivation. Tanshenosde Ⅰ in wild Taidangshen is also lower than that in cultivated Taidangshen
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Fufanghuangbai Liquid is a compound prescription for topical use containing fructus Forsythiae, Cortex phellodendri and Flos Lonicerac as its main ingredients.A method for the identification of these ingredient by TLC was developed while the content of berberine hydrochloride in the prescription was determined with column chromatography and TLC scanning.The method can be used to control the quality of Fufanghuangbai Liquid.
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AIM:To determine gentianaceae and jasminoidin in Longhui Capsules (Radix et Rhizoma Gentia-nae, Aloe, Fructus Gardeniae, etc.) METHODS: The Waters Symmetry C_ 18 column (4.6 mm?150 mm, 5 ?m) was used with mobile phase of methanol-water (17∶83). The flow rate was 1.0 mL/min. The detecting wavelength was at 254 nm, and the column temperature was at 30 ℃. RESULTS: The methodological study showed that a good linear correlation existed in the range of 60.72-454.4 ng for gentianaceae and the range of 96.32 -722.40 ng for jasminoidin. The average recovery of gentianaceae was 99.57%(n=9) and RSD was 1.27% , jasminoidin was 99.10%(n=9) and RSD was 2.30%. CONCLUSION: The method is simple, accurate and can be used for quality control of the preparation.
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AIM: To establish a simple and rapid method for the quality control of Chinese traditional medicine. METHODS: Pericarpium Citri Reticulatae,Fructus Psoraleae,Rhizoma Atractylodis macrocephalae and Radix Codonopsis in Wenweishu Tablets were identified by TLC at two thin-layer chromatographic plates.The contents for hesperidin,psoralen and isopsoralen in this preparation were determined simultaneously by HPLC. RESULTS: The methodological study showd that a good linear correlation existed in the range of 0.039 6-0.495 ?g(r=(0.999 99)) of hesperidin,0.018 5-0.231 ?g(r=0.999 98) of psoralen and 0.019 7-0.246 2 ?g(r=0.999 91) of isopsoralen applied respectively.The average recovery of hesperidin was 98.42%(n=9),RSD was 2.69%;The average recovery of psoralen was 98.29%(n=9),RSD was 1.08%.The average recovery of isopsoralen was(98.93%)(n=9),RSD was 2.41%. CONCLUSION: The method is simple、rapid and practicle.
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AIM: To establish a method for the quality control of Danqi Tablet(Radix et Rhizoma Salviae Miltiorrhizae,Radix et Rhizoma Notoginseng,etc). METHODS: The content of salvianic A and protocatechualdehyde,notoginsenoside R_1 and ginsenoside Rg_1 in Danqi Tablet were determined simultaneously respectively by HPLC. RESULTS: The methodological study showed that a good linear correlation existed in the range of 0.164-3.28 ?g(r=0.999 99) of sodium salvianic A,0.013-0.52 ?g(r=0.999 999) of protocatechualdehyde,0.2-6 ?g(r=0.999 997) of notoginsenoside R_1 and 0.824-24.72 ?g(r=0.999 96) of ginsenoside Rg_1,respectively.The average recovery of sodium salvianic A was 98.34%(n=9),RSD was 2.03%,100.65% for protocatechualdehyde(n=9),RSD was 2.96%,98.97% for notoginsenoside R_1(n=9),RSD was 2.81%;98.93% for ginsenoside Rg_1(n=9),RSD was 0.57%. CONCLUSION: The determination methods are simple,accurate and reproducible.The method can be used for quality control of Danqi Tablet.
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AIM: To establish a simultaneous quantitative method which was simple and rapid for mixture of ephedrine、 D-pseudo-ephedrine and amygdalin. METHODS: Ephedrine、D-pseudo-ephedrine and amygdalin in Herba Ephedrae、Semen Armeniacae Amarum、Zhisou Lixiao Tablets、Zhisou Lixiao Capsules and its semi-finished products were determinated by HPLC. RESULTS: The methodological study showed that a good linear correlation existed in the range of 0.039 68-0.892 8 ?g for ephedrine hydrochloride and 0.106 3-4.252 ?g for amygdalin, respectively. The average recovery of ephedrine hydrochloride was 98.2%(n=9) and RSD was 1.6%; The average recovery of amygdalin was 99.4%(n=9) and RSD was 2.4%. CONCLUSION: This method is simple, accurate and reproducible and with high precision. The method can be used for quality control of Chinese preparations.
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Objective: To establish the quality standard for Erxiaqingxin Tablets(Cordyceps, Rhizoma Pinelliae, Radix Puerariae, Caulis Bambusae in Taenia, Fructus Aurantii Immaturus, Pericarpium Citri Reticulatae, etc.). Methods:TLC was performed to identify Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus and Radix Puerariae. HPLC was used to determine the content of puerarin. Results:The study on the quality control showed that the characteristic of identification by TLC was distinct and hightly specific. The quantification method had the linear range of 0.04092～2.4552?g. The average recovery was 99.36% and RSD was 2.32%. Conclusion:The method for identification and quantification was simple, realizable and reproducible. It can be used effectively for the quality control of Erxiaqingxin Tablets.
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AIM: To establish a method for the quality control of Xiao'er Ganmao Granules(Flos Chrysanthemi, Radix Cynanchi Atrati, Cortex Lycii, etc.). METHODS: Radix Cynanchi Atrati, Cortex Lycii and Flos Chrysanthemi were identified by TLC. The forsythin content in Xiao'er Ganmao Granules was determinated by HPLC. RESULTS: The methodological study showed that a good linear correlation for the forsythin existed in a range of 4.76～588ng. The average recovery of forsythin ( n =9) was 98.7% and RSD was 1.7%. CONCLUSION: The method is simple, accurate and with good repeating and high precision, and can be used for quality control of Xiao'er Ganmao Granules.
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Objective: To establish quality standard for Xiaoerhuichun Pills(Radix Scutellariae Radix Paeoniae Rubra, Rhizoma Coptidis, Folium Isatidis, Radix Puerariae, etc.). Methods: Radix Scutellariae, Radis paeoniae Rubra, Rhizoma Coptidis, Folium Isatidis and Radix Platycodonis were identified by TLC. The content for berberine hydrochloride in Xiaoerhuichun Pills was determined by TLCS canning. Results: The methodological study showed that a good linear correlation in the range of 0.05776 to 0.34656?g of the berberine hydrochloride. The average recovery of berberine hydrochloride ( n =8) was 98.69% and RSD was 0.73%, respectively. Conclusion: The method is simple, accurate and with good repeatability and high precision. The method can be used for quality control of Xiaoerhuichun Pills.
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Objective: To establish the quality control method for ephedrine in Herba Ephedrae and its preparation Zhisoulixiao Tablets (Herba Ephedrae, Pericarpium Papaveris, Semen Armeniacae Amrum, Radix Glycyrrhizae, Gypsum Fibrosum, Semen Lepidii, Semen Raphani, etc.) and Zhisoulixiao Capsules. Methods:Ephedrine hydrochloride contents in Herba Ephedrae and its preparation Zhisoulixiao Tablets and Zhisoulixiao Capsules were determined by TLC scanning with a mixture of chloroform ethyl acetate methanol water(13∶40∶22∶10)as a developer and single wavelength reflection sawtooth scanning. Results: The methodological study showed that a good linear correlation in the range from 0.29112?g to 1.4556?g of the ephedrine hydrochloride applied. The average recovery of ephedrine hydrochloride in Herba Ephedrae, Zhisoulixiao Tablets and Zhisoulixiao Capsules was 78.74%( n=5 ),99.46%( n=5) and 99.14%( n=5) ,respectively. Conclusion: The method established is simple,accurate with good reproducible and high precision.The method can be used for the quality control of Herba Ephedrae and its preparation
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Objective:To determine synchronously protocatechuic aldehyde and danshensu in Compound Danshen Lozenge and Dropping Pills.Methods:HPLC method was used.Results:The average recoveries(n=5) were 100.47% for Danshensu of Compound Danshensu Lozenge(RSD=1.08%) and 99.27% for protocatechuic aldehyde(RSD=1.22%), respectively. And the average recoveries(n=3) were 100.40% for Danshensu of Compound Danshen Dropping Pills(RSD=0.65%) and 98.47% for protocatechuic aldehyde(RSD=1.76%).Conclusion:This method is simple, quick with a good separation.